LO-HG4 Anti-human IgG4 monoclonal antibody

“LO-HG4”Anti-human IgG4 monoclonal antibody New SYngular product from SYnAbs

SYnAbs (www.synabs.be) is proud to announce the launch of new high added value products: two monoclonals from rat-LOU species highly specific to human IgG4 subclass, without any cross reaction with other human isotypes.

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 15% of all plasma protein content. Humans express four types of immunoglobulin G, known as IgG1, IgG2, IgG3, and IgG4.

IgG4 present conformational differences with IgG1 Cc2 domain loops. While the overall domain structure is conserved, the conformation of BC and FG loops is different, and in IgG4, the FG loop folds away from the Cc2 domain. As the differences are very slight, it’s very difficult to create anti-IgG4 antibody that doesn’t cross-react with the other IgG classes.

We conjugated our anti-IgG4 to HRP probe since we wanted to be sure to have similar outcomes with unconjugated and conjugated antibodies.

Coating: Human IgG1, IgG2, IgG3 from Sigma, IgG4 from Abcam. 5 μg/ml.

LO-HG4-clone#1. 1mg/ml

Clone#1. dilution/coating	IgG1	IgG2	IgG3	IgG4
2000	0,090	0,100	0,080	2,590
4000	0,070	0,080	0,080	2,270
8000	0,070	0,070	0,060	1,900
16000	0,060	0,060	0,060	1,310
32000	0,060	0,060	0,060	0,850
64000	0,060	0,060	0,060	0,580
128000	0,060	0,060	0,070	0,360
256000	0,060	0,060	0,060	0,230
512000	0,060	0,060	0,060	0,150
0	0,060	0,060	0,060	0,090

LO-HG4-clone#5. 1 mg/ml

Clone#5. dilution/coating	IgG1	IgG2	IgG3	IgG4
2000	0,100	0,150	0,110	2,730
4000	0,080	0,110	0,090	2,400
8000	0,070	0,090	0,070	1,900
16000	0,070	0,070	0,070	1,320
32000	0,060	0,070	0,060	0,830
64000	0,060	0,060	0,060	0,500
128000	0,060	0,060	0,060	0,300
256000	0,060	0,060	0,060	0,200
512000	0,060	0,060	0,060	0,150
0	0,060	0,060	0,060	0,060

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