DNP, or 2,4-Dinitrophenol, is an organic compound with the chemical formula HOC₆H₃(NO₂)₂. Known for its yellow crystalline appearance and musty odor, DNP is a hydrophobic, water-soluble ligand that binds to transthyretin at acidic pH. It has unique properties, such as the ability to inhibit ATP production in cells with mitochondria by disrupting electron flow and the pumping of H⁺ ions for ATP synthesis. This makes DNP a valuable molecule in both biochemical research and in vitro diagnostic assays.

Dinitrophenol (DNP) plays a significant role in various scientific applications. It is most commonly used as a hapten, which is not found endogenously in tissues, making it an ideal candidate for use as a control in immuno-assays. Its ability to stimulate a strong immune response when conjugated with proteins or peptides has made it an invaluable tool in numerous research applications. Some key applications of DNP include:

• Biotin-Free Systems: DNP serves as an excellent alternative to biotin in assays, where biotin-free systems are preferred. Probes or antibodies labeled with DNP can be detected using anti-DNP monoclonal antibodies from SYnAbs.

• Oxidative Damage Detection: DNP can be used to visualize and localize oxidative damage in tissues. This is achieved through immunodetection of DNP adduction, resulting from the reaction of the tissue with DNPH.

• Neurodegenerative Disorder Research: DNP has been linked to potential therapies for neurological conditions, making it relevant in the study of neurodegenerative diseases.

• Environmental & Industrial Uses: DNP continues to be used in pesticides, fertilizers, wood preservatives, and even explosives.

Anti-DNP monoclonal antibodies are essential for several research and diagnostic applications. These antibodies serve as powerful tools in the study of DNP-related reactions and are particularly useful in in vitro diagnostic (IVD) kits. Some key uses include:

• Isotype Control: Anti-DNP antibodies are commonly used as non-targeting isotype controls in both in vitro and in vivo studies. These antibodies are essential for establishing baseline readings and ensuring accurate results when evaluating experimental data.

• Negative Control: They are also widely used as irrelevant monoclonal antibodies for research use in assays like ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. By binding to non-specific targets, they help control for potential background signal interference, ensuring that your data reflects true interactions and not non-specific binding.

• Diagnostic and Therapeutic Research: Anti-DNP antibodies are integral components of diagnostic assays, particularly in the pharmaceutical and IVD industries, where precise detection is critical.

• Replacing biotin/streptavidin detection system by DNP/anti-DNP monoclonals

SYnAbs has developed a wide range of monoclonal antibodies targeting DNP, including 16 different antibody references in both mouse (MA-DNP) and rat (LO-DNP) species. The rat-derived LO-DNP antibodies, in particular, have been recognized for their superior immune response, which is crucial for the development of high-performance antibodies. As a result, these LO-DNP antibodies are highly regarded in the pharmaceutical and in vitro diagnostic kit industries.

The LO-DNP-2 antibody from SYnAbs ranks among the top-selling references, offering researchers a reliable and validated tool for their studies. These antibodies are developed and optimized for use in key assays such as Western blot, ELISA, and immunohistochemistry, ensuring accurate and reproducible results in research and diagnostics.

• Validated Performance: SYnAbs' DNP antibodies have been extensively validated by customers in multiple applications, including immunohistochemistry, ELISA, and Western blot.

• Species-Specific Options: With antibodies available in both mouse and rat species, SYnAbs offers flexibility depending on the immune response required for specific experimental conditions.

• Robust for Pharma & IVD: SYnAbs' anti-DNP antibodies are widely used in the pharmaceutical and IVD industries for the development of precise in vitro diagnostic kits and in high-throughput screening.

• SYnAbs anti-DNP monoclonal antibodies have been cited in many publications including

1. Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle
2. Monitoring Autophagy in Alzheimer’s Disease and Related Neurodegenerative Diseases
3. Paired Activating and Inhibitory Immunoglobulin-like Receptors, MAIR-I and MAIR-II, Regulate Mast Cell and Macrophage Activation
4. TA-270[4-hydroxy-1-methyl-3-octyloxy-7-sinapinoylamino-2(1H) quinolinone], an anti-asthmatic agent, inhibits leukotriene production induced by IgE receptor stimulation in RBL-2H3 cells
5. Cytochemical demonstration of oxidative damage in Alzheimer disease by immunochemical enhancement of the carbonyl reaction with 2,4-dinitrophenylhydrazine
6. Aging-associated shifts in functional status of mast cells located by adult and aged mesenteric lymphatic vessels